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. 2017 Nov 17;85(12):e00591-17. doi: 10.1128/IAI.00591-17

FIG 1.

FIG 1

Binding of anti-OmpA MAbs to rOmpA and A. baumannii ATCC 19606. (A) Purified anti-OmpA MAbs showed positive binding to rOmpA compared to preimmune serum based on ELISA. Postimmune serum was used as a positive control. (B) Michaelis-Menten profiles of binding of anti-OmpA MAbs to rOmpA. (C and D) Anti-OmpA MAbs showed strong binding to formalin-killed ATCC 19606 using cell-based ELISA (C) and to live ATCC 19606 via flow cytometry (D) compared to antibody isotype controls (2C11 versus IgG2a; 5F8, 7D4, 10B9, and 12E11 versus IgG1). n = 3. **, P < 0.01; ****, P < 0.0001 (one-way ANOVA followed by Dunnett's multiple comparisons). The data are presented as means ± standard deviations. PE-A, phycoerythrin area.