FIG 5.

Fluorescence microscopy of HTB-9 cells incubated with recombinant H03-IPSE (NLS, SKRRRKY), H06-IPSE (NLS, SKRGRKY), or the H03-IPSE mutant (NLS, SKAAAKY). HTB-9 cells were stained with Hoechst 33342 nuclear stain for 15 min at room temperature, followed by staining with a mouse anti-His antibody and Alexa Fluor 555-conjugated goat anti-mouse IgG(H+L) as a secondary antibody. The right column shows the overlay of the two channels. The primary anti-His antibody was omitted in the control lane. Bar, 100 μm.