Stage-specific expression of H-IPSE mRNA. Shown are RT-PCR results for H-IPSE obtained from cDNAs prepared by reverse transcription of DNase-treated RNAs isolated at various life stages of S. haematobium. Ladder, 100-bp DNA ladder; egg, S. haematobium egg cDNA; mir, miracidial cDNA; cer, cercarial cDNA; som, in vitro mechanically transformed schistosomulum cDNA; AdF, AdM, and Ad mix, mixed cDNAs from female, male, and mixed adult worms, respectively; ShTub, S. haematobium tubulin (control housekeeping gene).