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Amplification of the cDNA of the gene tia. The expression of the gene tia by strain ED32 (A) and by the K-12 strain JM109/pGEM_tia (B) was evaluated by reverse transcriptase PCR amplification of a 571-bp fragment within the coding sequence with the primers tiaUP and tiaLO (4) using the same amount of cDNA from the two strains as the template.
