FIG 3.
CATH-2 inhibits LPS-induced macrophage activation. (A) P. aeruginosa (3 × 106 cells/ml) was incubated for 0.5 h with 250 μg/ml gentamicin, followed by the addition of 5 μM CATH-2, LL-37, or eCATH-1. These mixtures were used for stimulation of J774.A1 cells for 2 h, after which TNF-α release was determined. Statistical differences were determined by one-way ANOVA with Dunnett's post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 4. Error bars = SEM. (B and C) J774.A1 cells were stimulated with P. aeruginosa LPS (100 ng/ml) (B) or P. aeruginosa flagellin (10 ng/ml) (C) in combination with CATH-2 or LL-37, after which TNF-α release was determined after 2 h. n = 3. Error bars = SEM.