FIG 5.
Effect of CATH-2-mediated killing on proinflammatory cytokine release in vivo. Male C57BL/6 mice were instilled with 50 μl of 2 × 106 CFU/ml P. aeruginosa, which was either CATH-2 killed (20 μM), gentamicin killed (1 mg/ml), or heat killed (90°C, 1 h). Alternatively, control mice were instilled with an air bolus. After 6 h, TNF-α (A), IL-6 (B), IL23p19 (C), IL-12p70 (D), G-CSF (E), KC (F), MIP-2 (G), and IL-33 (H) were determined by Luminex technology. n = 5 or more. Error bars = SEM. Statistical differences were determined by one-way ANOVA with Bonferroni's post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.