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. 2017 Nov 17;85(12):e00297-17. doi: 10.1128/IAI.00297-17

FIG 1.

FIG 1

Neutrophil reactive oxygen species (ROS) production in response to periodontal bacteria. Neutrophil total ROS (A), extracellular ROS (B), and superoxide (C) production levels were quantified (right), and their levels of production in response to periodontal bacteria were assayed using luminol, isoluminol, and lucigenin enhanced chemiluminescence, respectively, over a time course of 130 min (left). ROS release in response to PBS (unstimulated negative control), phorbol 12-myristate 13-acetate (PMA; 25 nM; positive control), and opsonized S. aureus (positive control) was also quantified. Data are presented as relative light units (RLU) and represent the results for neutrophils from five different donors assessed in triplicate wells. *, P < 0.05; **, P < 0.01; ***, P < 0.001. A.a., A. actinomycetemcomitans; A. actinomyc a, A. actinomycetemcomitans serotype a; A. actinomyc b, A. actinomycetemcomitans serotype b; F. nuc. subsp. nuc. and F. nucleatum n, Fusobacterium nucleatum subsp. nucleatum; F. nuc. subsp. polym. and F. nucleatum p, Fusobacterium nucleatum subsp. polymorphum; Ops., opsonized.