Table 1.
Gene | Gene Primer sequence |
---|---|
ALP | Forward 5'-GGACCATTCCCACGTCTTCAC-3' Reverse 5'- CCTTGTAGCCAGGCCCATTG-3' |
RUNX2 | Forward 5'-CACTGGCGCTGCAACAAGA-3' Reverse 5'-CATTCCGGAGCTCAGCAGAATAA-3' |
OCN | Forward 5'-CCCAGGCGCTACCTGTATCAA-3' Reverse 5'-GGTCAGCCAACTCGTCACAGTC-3' |
Fibronectin | Forward 5'- CACCCAATTCCTTGCTGGTATC-3' Reverse 5'- TATTCGGTTCCCGGTTCCA-3' |
Integrin β1 | Forward 5'- GTGAGTGCAACCCCAACTACACT-3' Reverse 5'- AAGGCTCTGCACTGAACA CATTC-3' |
COL-I | Forward 5'- CCAGAAGAACTGGTACATCAGCAA-3' Reverse 5'- CGCCATACTCGAACTGGAATC-3' |
BSP | Forward 5'-GGGCAGTAGTGACTCATCCGA-3' Forward 5'-TCTTCATTGTTTTCTCCTTCATTTG-3' |
OPN | Forward 5'-TCTGGGAGGGCTTGGTTGTC-3' Forward 5'-TTTCCTTGGTCGGCGTTTG-3' |
TGF-β | Forward 5'-CACGTGGAGCTGTACCAGAA-3' Reverse 5'-CCGGTAGTGAACCCGTTGAT-3' |
β-actin | Reverse 5'-TGGCACCCAGCACAATGAA-3' Reverse 5'- CTAAGTCATAGTCCGCCTAGAAGCA-3' |