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(a) Immunohistochemical analysis of caspase-3, cleaved caspase-3, and HIF-1α after pretreatment with PBS, free Ce6, Ce6+Catalase mixture, and C&C-Ce6 nanoparticles (×400); (b) immunofluorescence staining with Tunnel of tumor slides from CAL-27 tumor-bearing mice pretreated with C&C-Ce6, free Ce6, catalase/Ce6 mixture, and PBS and irradiated with 650 nm laser. Tunnel-positive cells (green); DAPI (blue).
