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. 2017 Oct 13;7(18):4537–4550. doi: 10.7150/thno.19546

Figure 9.

Figure 9

(a) Representative results of flow cytometric analysis of cell apoptosis of CAL-27 cells treated with C&C-Ce6 nanoparticles, free Ce6, Catalase+Ce6, Chitosan+Ce6 nanoparticles, and PBS, followed by staining with Annexin V-FITC/PI. (Q4: living cells; Q3: early apoptotic cells; Q2: late apoptotic cells; Q1: necrotic cells. Numbers in the profiles indicate the percentage of the cells present in the area); (b) Caspase-3 protein activity, (c) Western blot photograph of caspase-3 and cleaved caspase-3; (d) Western blot analysis of caspase-3 in CAL-27 cells pretreated with C&C-Ce6 nanoparticles, free Ce6, Catalase+Ce6, Chitosan+Ce6 and PBS.