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. 2017 Oct 13;7(18):4566–4576. doi: 10.7150/thno.19840

Figure 3.

Figure 3

(A, B) MeR/PhR stabilize p53 and elevate protein levels of p53 and its targets MDM2 and MDMX. Log-phase PA-1 and MCF-7 cells were incubated with 40 µM peptides (MeR/S, PhR/S) or 1 µM nutlin-3a, and cell lysates were analyzed by Western blotting. (C, D) PhR shows induction of p53 and p53 target genes in p53wt cell lines. Exponentially growing p53wt cancer cell lines were incubated with 40 µM peptides or 1 µM nutlin-3a for 48 h, and mRNA level of p53 and p53 targets MDM2, MDMX, and MIC or stemness-related genes were analyzed by quantitative PCR and expressed as fold increase. Error bars represent SEMs for triplicates of three independent experiments, **, P<0.01. *, P<0.05.