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. 2017 Oct 13;7(18):4577–4590. doi: 10.7150/thno.20005

Figure 3.

Figure 3

Overexpression of SVV attenuates cardiomyocyte apoptosis. A, Effect of overexpressing SVV in hypoxia-induced cardiomyocyte apoptosis. B, Quantification of TUNEL positive CM. **, P<0.01. C, Sequence of mutant SVV-T34A (T34A). D, Western blotting showing phosphorylation level of SVV-T34. ad, adenovirus. E, Co-immunoprecipitation of CM overexpressing SVV with caspase-9. F, Co-immunoprecipitation of CM overexpressing T34A with caspase-9. G, Western blotting showing that overexpressing SVV attenuates caspase-3 activation but not T34A. Dox, doxorubicin. H, TUNEL staining indicating apoptotic cardiomyocytes in the hearts with overexpressing vehicle, SVV, T34A, or NLS-SVV after MI. NLS, nuclear localization signal. White bar, 50 μm; yellow bar, 10 μm. I, Quantification of apoptotic CM at the border zone. *, P<0.05. J, Western blot showing caspase-3 activation.