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. 2017 Oct 13;7(18):4577–4590. doi: 10.7150/thno.20005

Figure 5.

Figure 5

Overexpression of NLS-SVV enhances cardiomyocyte proliferation. A, Immunofluorescence staining revealing localization of SVV in neonatal mouse CM overexpressing SVV or NLS-SVV. NLS, nuclear localization signal; adNLS-SVV, adenovirus carrying NLS-SVV. B, Western blotting showing SVV expression in the cytoplasm or the nucleus fraction. C, Co-immunoprecipitation indicating the interaction of SVV and Aurora B in NLS-SVV-overexpressing CM. D, Quantification of CM number after overexpressing NLS-SVV. AZD1152, 10nM. *, P<0.05. E, Western blotting showing histone 3 phosphorylation (H3P) level in CM overexpressing NLS-SVV. F, Immunofluorescence staining indicating H3P positive cardiomyocytes at the border zone. Scale bar, 20 μm. G, Quantification of H3P-positive CM at the border zone. *, P<0.05. H, Western blotting showing H3P phosphorylation level after overexpressing NLS-SVV in vivo.