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. 2017 Nov 15;10:369. doi: 10.3389/fnmol.2017.00369

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Copyright © 2017 Lizen, Moens, Mouheiche, Sacré, Ahn, Jeannotte, Salti and Gofflot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-qPCR analysis of selected candidate target genes of HOXA5 in the medulla oblongata (A) and pons (B) of tamoxifen-treated Hoxa5^flox/flox;CMV-CreER^T2-/- (Hoxa5 control; dark blue) and tamoxifen-treated Hoxa5^flox/flox;CMV-CreER^T2+/- (Hoxa5 cKO; light blue) at P21. Data are reported as means ± SEM. Asterisks depict statistically significant differences compared with Hoxa5 control brains: ^∗∗∗p < 0.001, ^∗∗p < 0.01, and ^∗p < 0.05. p-Values are from ANOVA test and are provided in Table 1. n = 3–6 for Hoxa5 control brains, n = 3–8 for Hoxa5 cKO brains. The relative transcripts abundance is calculated using the 2^-ΔΔCt method as detailed in Section “Materials and Methods,” where normalized values measured in Hoxa5 cKO are reported to normalized values measured in Hoxa5 control. The fold change is presented as a percentage of this value: (2^-ΔΔCt) × 100.