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. 2017 Jun 27;4(4):563–575. doi: 10.1002/ehf2.12183

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© 2017 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression and distribution of non‐polyaminated lipocalin‐2 (npLcn2) protein in the pericardium tissue biopsies collected from Danish patients during cardiothoracic surgery. (A) Immunohistochemical staining was performed for pericardium tissue sections using polyclonal antibodies against npLcn2. (B) Samples were stratified by the pattern of npLcn2 expression and distribution in pericardium tissue sections. Note that the average left ventricular ejection fraction (LVEF) of patients in Group III was significantly lower than that of Groups I and II. Arrows indicate the positive staining of npLcn2. A = adipocytes, M = mesothelial cells, and L = leucocytes. Magnification, ×200. (C) Based on the number of positively stained cells and the distribution pattern, samples were separated into three groups for comparison of their lipocalin‐2, total cholesterol, and plasma creatinine levels. *P < 0.05 (n = 9–12).