FIGURE 3.
Tumor reactive lymphadenopathy. A, Normal thyroid parenchyma, tracheal cartilage (TC), and caudal (CA) deep cervical lymph node in a wild-type (WT) mouse (original magnification ×40). B, Cranial (CR) deep cervical lymph node draining a high-grade papillary thyroid carcinoma (PTC) tumor with extrathyroidal extension in a BrafV600E/TPO-Cre mouse (original magnification ×100). C, Cranial deep cervical lymph node draining an intrathyroidal PTC in a BrafV600E/TPO-Cre mouse (original magnification ×200). D, Caudal deep cervical lymph node with a subcapsular sinus (SCS) width measuring 8.6 μm in a wild-type mouse (original magnification ×200). E, Cranial deep cervical lymph node with a SCS measuring 41.3 μm in a BrafV600E/Pten+/−/TPO-Cre mouse (original magnification ×200). SCS is measured in micro-meters (black bars) in D and E. F, Cranial deep cervical lymph node demonstrating subcapsular histiocytes in a BrafV600E/Pten+/−/TPO-Cre mouse (original magnification ×100). G, Popliteal lymph node in an 8-week-old wild-type mouse (original magnification ×100). H, Popliteal lymph node in a 3-week-old BrafV600E/TPO-Cre mouse (original magnification ×100). I, Tumors in mice with oncogenic BrafV600E expression with and without Pten insufficiency had larger SCS widths compared with wild-type animals. Each dot represents an individual lymph node. Student’s t test, *P <.05. All sections were stained with hematoxylin-eosin [Color figure can be viewed at wileyonlinelibrary.com]