Table 1. Fluorescence dye- coupled lectins for cell surface staining of S. purpuratus coelomocytes.
| Name | Description | Main specificitiesa | Fluorophore |
|---|---|---|---|
| sWGA | Wheat Germ agglutinin, succinylated | oligosaccharides containing terminal N-acetylglucosamine or chitobiose; sialic acid residues are not bound | rhodamine |
| LCA | Len culinaris lectin | α-linked mannose residues; an α-linked fucose residue attached to the N-acetylchitobiose of the core oligosaccharide significantly enhances affinity | rhodamine |
| PSA | Pisum sativum agglutinin | α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence; calcium and manganese ions are required for binding | rhodamine |
| GSL-1 | Griffonia simplicifolia lectin I | Galactose | rhodamine |
| PHA-L | Phaesolus vulgaris leucoagglutinin | complex structures | rhodamine |
| PHA-E | Phaesolus vulgaris erythroagglutinin | complex structures | rhodamine |
| SJA | Saphora japonica agglutinin | carbohydrate structures terminating in N-acetylgalactosamine and galactose residues, with preferential binding to β anomers | rhodamine |
| PNA | Peanut agglutinin | Galactose | fluorescein |
| ECL | Erythrina cristagalli lectin | Galactose | fluorescein |
| Jacalin | Jacalin | Galactose | fluorescein |
| STL | Solanum tuberosum lectin | oligomers of N-acetylglucosamine and some bacterial cell wall oligosaccharides containing N-acetylglucosamine and N-acetylmuramic acid; similar but not identical specificities as WGA and DSL | fluorescein |
| LEL | Lycopersicon esculentum lectin | shares some specificities with STL, DSL, and WGA, but has been reported to be dissimilar in many respects; binds to glycophorin and Tamm-Horsfall glycoprotein and labels the vascular endothelium in rodents | fluorescein |
| DSL | Datura stramonium lectin | (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue | fluorescein |
| GSL-2 | Griffonia simplicifolia lectin II | N-acetylglucosamine | fluorescein |
| VVL | Vicia villosa lectin | α- or β-linked terminal N-acetylgalactosamine, in particular a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen) | fluorescein |
aThe information about the binding specificities was obtained from Vector Laboratories (https://vectorlabs.com).