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. 2017 Nov 10;12(11):e0187987. doi: 10.1371/journal.pone.0187987

Table 2. Oligonucleotides to test gene expression.

Gene	Name	Nucleotide sequences (5’–3’)	Cell fraction^a
Sp-Rpl39	SPU_18884QF	`AGGACAGGCAACAGGATTCG`	all
Sp-Rpl39	SPU_18884QR	`TGAGTCTGGGCTGATGCTTG`	all
Sp-Egr	WHL22.280477F	`CATCGCCGCACCACAATATG`	ph^b
Sp-Egr	WHL22.280477R	`GGATAACTGCTGGGTCCTCG`	ph^b
Sp-FoxJ1	WHL22.468365F	`TCAAGAAGAGACGACACGCC`	vcs^c
Sp-FoxJ1	WHL22.468365R	`TGTGGTGCTTGGTATCGTCC`	vcs^c
Sp-Giant	WHL22.668554F	`CATACACCGAGCCGACACTG`	rs^d
Sp-Giant	WHL22.668554R	`TTTCAGCGACGGGGTAGTTC`	rs^d
Sp-B7L3	SPU20457QF	`TTCGGCTGCCTAACGAAACT`	ph
Sp-B7L3	SPU20457QR	`CACCAGGAGAAACACGACGA`	ph
Sp-P2rx4	SPU19489F	`GTTAAACTTCGCATCGGGCG`	vcs
Sp-P2rx4	SPU19489R	`TGTCTTCTGCGGTCCATTCTC`	vcs
Sp-Pks1	SPU_002895Q2F	`GCAAGCAGTTCAAGGCTCAG`	rs
Sp-Pks1	SPU_002895Q2R	`TTGTCTTCCCATGTTGCCGT`	rs

^afractions were obtained by density gradient fractionation using a step gradient

^bphagocytic cells

^cmix of vibratile and colorless spherule cells

^dred spherule cells