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Proliferation of HEL-specific T cells in response to recombinant Ab with the HEL-(46–61) epitope. 3A9 T hybridoma cells were cultured together with irradiated C3H spleen cells and titrated amounts of recombinant Ab and synthetic HEL peptide. αIgD.L6-HEL (▴), αNIP.L6-HEL (♦), αIgD.wt (■), and synthetic HEL-(46–61) peptide (✠). IL-2 production by 3A9 cells was measured as incorporation of [3H]dThd into CTLL-2 cells.
