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The activity assays for tA16ACII and CS ABC. CS-A and CS-B (DS) were used as substrates for the same protein concentration of tA16ACII and CS ABC expressed in E. coli. Assay were carried out in a quartz crystal cuvette at 37 °C and each consisted of 50 μL of 20 mg/mL CS-A or CS-B, 640 μL of digestion buffer (50 mM ammonium acetate, pH 7.4), and 10 μL of the same concentration of tA16ACII or CS ABC. Absorbance was measured at 232 nm.
