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. 2017 Nov 8;12(11):e0186262. doi: 10.1371/journal.pone.0186262

Fig 4. SpiHIF promotes human HRE transcriptional activation.

Fig 4

HEK293 were transfected either with luciferase reporter plasmid together with empty, spiHIFα, spiHIFβ, or a combination of both spiHIF expression vectors. In all cases, a control Renilla expression vector was co-transfected to normalize for transfection efficiency. 24h after transfection, cells were maintained under normoxic or hypoxic conditions in an anaerobic workstation for 24h. Cells were lyzed and firefly and renilla luciferase activity were measured. A ratio of firefly/renilla luciferase activity is shown. Results are representative of three independent experiments performed in triplicate. Values are means ± SD (n = 3). * P< 0.05.