Table 2. Antibodies, concentration and antigen retrieval conditions for immunohistochemistry.
All reagents were from Dako unless otherwise specified. Visualisation of antigen: antibody complexes was performed using the DAB+ liquid Substrate chromogen system (K3467).
| Antigen | Antibody | Species reactivity | Retrieval | Primary antibody conc. | Secondary antibody |
|---|---|---|---|---|---|
| Oas2 | M18, sc49858 Santa-Cruz | Mouse/ human | pH9 S2367 Pressure Cooker 15secs | 1:200 | Goat Immpress-HRP (Vector Labs) MP7405 |
| Anti Milk | Accurate Chemical & Scientific CO. YNRMTM | mouse | pH6 S1699 Waterbath 20 mins | 1:12000 | Envision Rabbit (K4009) |
| Cleaved Caspase 3 | Asp175 5A Cell Signaling 9661 | Mouse/ human | pH9 S2367 Pressure Cooker 30secs | 1:100 | Envision Rabbit (K4009) |
| BrdU | Bu20a M0744 Dako | mouse | pH9 S2367 Waterbath 20 mins | 1:100 | Envision Mouse (K4007) |
| P-Stat1 | Tyr701 (58D6) Cell Signalling 9167 | mouse | pH9 S2367 Pressure Cooker 30secs | 1:800 | Signal Stain Boost Cell Signalling 8114 |
| P-Stat5 | Tyr694 (C11C5) Cell Signalling 9359 | mouse | pH9 S2367 Pressure Cooker 30secs | 1:600 | Signal Stain Boost Cell Signalling 8114 |