Fig 4. Phylogenetic analysis of partial sequences of the pol s region (alignment of 107 amino acids) directly obtained from the metagenomic data of pooled fecal samples of M. molossus with other representative members of the Spumavirus genus.

The tree was inferred using the Bayesian method with the WAG + G model. Sequence identifiers include the NCBI accession number and the isolate name. The blue arrows indicate the sequence of bat-sourced spumavirus obtained in the present study. Posterior probabilities of the Bayesian analysis (>50%) are shown next to each node. The scale bar indicates amino acid substitutions per site.