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. 2017 Oct 23;13(10):e1007048. doi: 10.1371/journal.pgen.1007048

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© 2017 Salehi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — In situ hybridization showing the expression pattern of Nrp1 (A-C) and Sema3a (D-F) in mice at time points E13.5, E16.5, E18.5, and P1. Nrp1 was not expressed at E16.5 (A), weakly expressed in the SG and SV at E18.5 (B), and more highly expressed in the SG, greater epithelial region (GER), and SV at P1 (C). Sema3a expression pointed by arrows on the abneural side of the cochlear epithelium was observed around E13.5 (D) and continued at E16.5 (E) and E18.5 (F) on the same region of the cochlear epithelium and spiral ganglion neurons. (G, H) Negative controls for Nrp1 (E18.5) and Sema3a (E16.5) using sense probes. (I) Panel I shows the plane of sections. SG: spiral ganglion. SV: stria vascularis. Scale bar = 50 μm.