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. 2017 Oct 23;13(10):e1006691. doi: 10.1371/journal.ppat.1006691

Fig 2. Deletion of the central repeat region of aLANA restores OVA-peptide presentation.

Fig 2

(A) Schematic description of aLANA expression constructs. The full central repeat region was truncated from aLANA to generate a ΔCR-SIIN construct. (B) Representative results from flow cytometry analysis of SIINFEKL presentation on 293Kb cells 48h after transfection with the indicated constructs. Numbers in red boxes indicate percent of H-2Kb-SIINFEKL-positive cells within eGFP+ cells. (C) Percent of eGFP+ cells expressing H-2Kb-SIINFEKL complexes at the cell surface based on the analysis in B. (D) β-galactosidase activity in B3Z after overnight co-culture with 293Kb cells 48h after transfection with the indicated constructs. Bars show mean ± S.D. (n = 3). Statistical analyses by one-way ANOVA and Dunnett’s post-test with aLANA-SIIN as control comparison mean (**p≤0.01, ***p≤0.001).