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. 2017 Oct 23;13(10):e1006690. doi: 10.1371/journal.ppat.1006690

Fig 6. In vivo administration of lethal toxin decreased IL-22 and GM-CSF production in ILC3s.

Fig 6

Rag1-/- mice were administered control solution or 100 μg of lethal toxin by tail vein injection. Two days post-injection, mice were euthanized and (A) the percentage and total number of ILC3s in livers, lungs and spleens from control mice (open circles) or lethal toxin treated mice (closed squares) were determined by flow cytometry. (B-C) Isolated lymphocytes were unstimulated (open circles) or stimulated with IL-23, PMA and ionomycin (black squares) in the presence of brefeldin A for 5 hrs. IL-22 and GM-CSF production was determined by intracellular flow cytometry. Shown is a representative experiment of 2 experiments with 3 mice per group. * p≤0.05, ** p≤0.01, *** p<0.001, **** p<0.0001 and non-significant (ns) p>0.05 by Mann-Whitney test (A) and two-way ANOVA corrected for multiple comparison using Sidak’s multiple comparison test (C).