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. 2017 Dec;92(6):653–664. doi: 10.1124/mol.117.109959

Fig. 2.

Fig. 2.

(A) Calcium flux elicited by CFA (2.3 mg/ml per 180 μg/cm2), icilin (50 µM), and menthol (500 µM) on GCaMP6-overexpressing cells transiently transfected with either human TRPM8 wild type (WT; n = 47, 16, and 47 for icilin, menthol, and CFA, respectively) or the TRPM8 icilin and menthol binding site mutant Y745H (n = 3, 3, and 3 for icilin, menthol, and CFA, respectively). (B) Calcium flux elicited by CFA (2.3 mg/ml per 180 μg/cm2), icilin (50 µM), and menthol (500 µM) on GCaMP6-overexpressing cells transiently transfected with human TRPM8-WT (n = 47, 16, and 48 for icilin, menthol, and CFA, respectively), the PIP2 binding site mutant R1008Q (n = 3, 3, and 3 for icilin, menthol, and CFA, respectively), or adjacent residue L1009R (n = 3, 3, and 3 for icilin, menthol, and CFA, respectively). Calcium flux responses were first subtracted for control vector (CV) responses and then normalized to responses of the TRPM8-WT cells. Values represent the mean ± S.E.M. Statistically significant responses using two-way analysis of variance and post-testing using the Bonferroni multiple comparisons test relative to TRPM8-WT are represented as *P < 0.05 and ***P < 0.001.