Fig. 5.

(A) IL-8 mRNA quantification in BEAS-2B cells, TRPM8 shRNA- (TRPM8-KO), and scramble shRNA-overexpressing cells treated with CFA (0.34 and 0.68 mg/ml per 90 and 180 µg/cm²) for 24 hours at 37°C. Data are represented as the mean ± S.E.M (n = 3). Statistically significant responses using two-way analysis of variance and post-testing with the Bonferroni multiple comparisons test relative to vehicle are represented as ***P < 0.001 or relative to BEAS-2B wild type and scramble cells as †††P < 0.001. CXCL1 (B) and IL-8 (C) mRNA quantification in NHBE cells treated with CFA (0.68 mg/ml per 180 μg/cm²) and CFA+AMTB (2 µM) or CFA+LJO-328 (20 μM) for 24 hours at 37°C. CXCL1 and IL-8 mRNA expression in both cell types was normalized to the reference gene β2 microglobulin and quantified using ΔΔCT relative to the vehicle control. Data are represented as the mean ± S.E.M (n = 3). Statistically significant responses using two-way analysis of variance and post-testing with the Bonferroni multiple comparisons test relative to vehicle are represented as ***P < 0.001 or relative to CFA as ††P < 0.001.