Fig. 6.

(A) Calcium flux in HEK-293 cells stably overexpressing either human TRPM8, mouse Trpm8, or rat Trpm8 and treated with icilin (20 µM), menthol (0.5 mM), CFA (2.3 mg/ml per 180 μg/cm²), CaO (2.3 mg/ml per 180 μg/cm²), or CaCl₂ (2.3 mg/ml per 180 μg/cm²). Data are normalized to hTRPM8-OE responses and represented as the mean ± S.E.M (n = 6). Statistically significant responses were determined using a one-way analysis of variance and post-testing with Bonferroni multiple comparisons test relative to hTRPM8-OE (***P < 0.001) or to CFA (†††P < 0.001). Quantification of Il-6 (B), Cxcl1 (C) and Cxcl2 (D) mRNA in lungs of wild-type (WT) C57BL/6 and Trpm8−/− mice treated via oropharyngeal aspiration (25 μl) with either ice-cold saline or CFA (10 mg/kg in saline) for 4 hours. Data are represented as the mean ± S.E.M. (n = 6). Statistically significant responses using two-way analysis of variance and post-testing with the Bonferroni multiple comparisons test relative to vehicle are represented as *P < 0.05 and **P < 0.01. No statistically significant differences were observed for comparisons between the saline and CFA groups as a function of genotype, indicated as P > 0.05. mTrpM8, mouse TrpM8; N.D., not determined; rTrpM8, rat Trpm8.