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. 2017 Dec;92(6):653–664. doi: 10.1124/mol.117.109959

Fig. 7.

Fig. 7.

(A) Amino acid sequence alignment of human (NM_024080), mouse (NM_134252), and rat TRPM8 (NM_134371). The alignment and text graphics were generated using Clustal Omega software (EMBL-EBI, Hinxton, UK; https://www.ebi.ac.uk/Tools/msa/clustalo/). (B) Calcium flux elicited by CFA (2.3 mg/ml per 180 µg/cm2) and menthol (500 µM) on GCaMP6-overexpressing cells transiently transfected with either wild-type (WT) human (menthol n = 35, CFA n = 32) or mouse (menthol n = 6, CFA n = 6) or the mouse-to-human mutants S921G (menthol, n = 14; CFA, n = 16), S927A (menthol, n = 17; CFA, n = 11), and S932T (menthol, n = 14; CFA, n = 10). Data are represented as the mean ± S.E.M. Statistically significant responses using two-way analysis of variance and post-testing with the Dunnett’s multiple comparisons test relative to mouse TrpM8-WT are represented as *P < 0.05. mM8WT, mouse TRPM8 wild type; mS921G, mouse S921G; mS927A, mouse S927A; mS932T, mouse S932T.