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. 2017 Sep 1;28(3):136–144. doi: 10.1089/humc.2017.029

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Copyright 2017, Mary Ann Liebert, Inc.

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Figure 2. — Similar gene marking levels in rhesus peripheral blood (PB) cells derived from steady state BM CD34⁺ cells, as compared with mobilized CD34⁺ cells. (a) To compare transduction efficiency in hematopoietic repopulating cells between BM CD34⁺ cells and mobilized CD34⁺ cells (n = 2, ZG06 and ZH21), equal numbers of CD34⁺ cells from each source were transduced with GFP or YFP-expressing lentiviral vectors on identical conditions. These cells were transplanted into autologous animals following 10Gy total body irradiation. (b) On average during ∼3 years after transplantation in both animals, we observed similar %GFP or YFP in all lineage cells derived from BM cells, as compared with that from mobilized cells (n.s. in all lineage cells). (c) We observed similar vector copy numbers in both granulocytes and lymphocytes derived from BM cells, as compared with that from mobilized cells (n.s. in both cells).