FIG. 2.

Overexpressing the H₂S synthase CBS-enhanced AMPK activation in BV2 microglial cells. (A) Western blot analysis of CBS in BV2 cells. Regardless of LPS stimulation, CBS protein levels were significantly elevated in BV2 cells that were transfected with the plasmids expressing CBS (n=3). *p<0.05, compared with vector-transfected cells without LPS stimulation (Vector); ^##p<0.01, compared with vector-transfected cells with LPS stimulation (vector+LPS). (B) CBS overexpression significantly increased H₂S concentration in the media of BV2 cell cultures (n=5). **p<0.01 compared with nontransfected control cells (Con) or cells transfected with the vector plasmids (Vector). (C) CBS overexpression significantly increased AMPK activation in BV2 cells without LPS stimulation (n=3). **p<0.01, compared with control cells (Con) or cells transfected with the vector plasmids (Vector). (D) CBS overexpression significantly increased AMPK activation in BV2 cells in the presence of LPS stimulation (n=3). **p<0.01, compared with vector-transfected cells without LPS stimulation (Vector); ^##p<0.01, compared with vector-transfected cells with LPS stimulation (Vector+LPS). CBS, cystathionine β-synthase.