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. 2017 Nov 20;12(11):e0187522. doi: 10.1371/journal.pone.0187522

Fig 1. Genetic variation effects growth inhibition by glyphosate.

Fig 1

A. Shikimate pathway produces the precursor for phenylalanine, tyrosine, tryptophan, para-Aminobenzoic acid (PABA), folic acid and Coenzyme Q10. The canonical target of glyphosate is EPSPS in plants and Aro1 is the yeast homolog of EPSPS. PABA and 4-hydroxylbenzoate can be converted to Coenzyme Q10. B. Serial dilution of genetically diverse yeast on rich media (YPD) with dilutions of glyphosate as indicated (1% vol/ vol is equivalent to 78 mM). C. Serial dilution of genetically diverse yeast on minimal media with glyphosate. Aromatic amino acids, tryptophan (W), tyrosine (Y) and phenylalanine (F) were added to YM plates to make WYF.