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A. Protein alignment of Aro1 from genetically diverse yeast strains. B. Serial dilutions of haploid aro1Δ in RM11, C. S288c, and D. YJM789, expressing different alleles of plasmid encoded ARO1 were grown on YPD and YM with and without glyphosate. Rows labeled empty have pGS36 plasmid with no ARO1. Parental strains with the endogenous ARO1 expressed from the chromosome labeled WT carry an empty plasmid (pGS36).
