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. 2017 Nov 20;12(11):e0187522. doi: 10.1371/journal.pone.0187522

Fig 5. Genetic Linkage analysis of glyphosate sensitivity in glyphosate on minimal media with and without aromatic amino acids.

Fig 5

A. Genetic linkage of sensitivity of YJM789 to 0.15% glyphosate in yeast minimal media supplemented with aromatic amino acids (WYF). LOD score (y-axis) was mapped across the yeast genome (x-axis). The LOD significant levels (alpha = 0.05) was 3.65 and was marked by a red dashed line. B. Genetic linkage of sensitivity of YJM789 to 0.15% glyphosate in yeast minimal media (YM). LOD score (y-axis) was mapped across the yeast genome (x-axis). The LOD significant levels (alpha = 0.05) was 3.47 and was marked by a red dashed line. C. The genomic loci under the peak located on chromosome 16 contains 14 genes. Genes encoded on the top strand are in yellow and genes encoded on the bottom strand are blue. D. Serial dilutions of S288c (GSY147), YJM789 and BY4741 with DIP5 knocked out grown on YM (HULM), WYF, aspartic acid (D) with glyphosate at the concentrations indicated. Histidine, uracil, leucine and methionine (HULM) were supplemented for growth of BY4741. E. Serial dilutions of S288c (GSY147) x YJM789 hybrids. The entire DIP5 coding region in either parent was deleted (Δ), crossed, and the resulting hemizygotes were grown on solid media YM, WYF, aspartic acid (D) with glyphosate at the indicated concentrations and supplemented.