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. 2017 Nov 20;12(11):e0187522. doi: 10.1371/journal.pone.0187522

Fig 6. Regulation of DIP5 by glyphosate and aspartic acid.

Fig 6

A. RNA expression levels of DIP5 mRNA in AWRI1631, RM11, YJM789 and S288c grown in YM with and without 0.25% glyphosate (RU). Q RT-PCR of DIP5 mRNA levels are normalized to ACT1 mRNA. B. Serial dilution of genetically diverse yeast on minimal media with glyphosate supplemented with aspartic acid. C. Different responses of dip5Δ yeast in liquid media supplemented with different aromatic metabolites and amino acids on exposure to glyphosate. BY4741 and GSY147 were grown in the presence of glyphosate (0.1% in HULM, WYF and D and 1% in YPD) and the optical density was measured in log phase (10 hr).