Table 3. Percent sequence identity of the mosquito genotypes to Rickettsia species with validly published names and other mosquito rickettsial strains provided in the GenBank.
| Gene | Mosquito pool | Mosquito species | Sequence length | Percent identity with the closest Rickettsia sp. (number of matches/sequence length) | Accession number | Fournier et al. (41) Cutoff values |
|---|---|---|---|---|---|---|
| rrs | A12.2638 | Culex pipiens | 1091 | 99.9% (1087/1088) R. bellii | CP000087 | ≥99.8% |
| 99.9% (806/807) R. bellii WHANSA-97_An. sinensis | KU586119 | |||||
| 98.1% (1006/1026) R. monacensis_Cx. quinquefasciatus | KU586120 | |||||
| 97.5% (384/394) Candidatus R. sp. An. sinensis | KU586118 | |||||
| 95.5% (362/379) Candidatus R. sp. Cx. tritaeniorhynchus | KU586123 | |||||
| A12.2646 | Mansonia uniformis | 1096 | 99.3% (1083/1091) R. bellii | CP000087 | ||
| A12.2639 | Culex pipiens | 1090 | 99.3% (1082/1090) R. bellii | CP000087 | ||
| A12.2784 | Culex pipiens | 1090 | 99.3% (1082/1090) R. bellii | CP000087 | ||
| A12.2856 | Aedes esoensis | 1091 | 99.3% (1083/1091) R. bellii | CP000087 | ||
| A12.3106 | Aedes esoensis | 1090 | 99.3% (1082/1090) R. bellii | CP000087 | ||
| A12.3271 | Aedes esoensis | 1419 | 99.3% (1409/1419) R. bellii | CP000087 | ||
| 99.3% (1093/1101) R. bellii WHANSA-97_An. sinensis | KU586119 | |||||
| 98.2% (1291/1314) R. monacensis_Cx. quinquefasciatus | KU586120 | |||||
| 97.5% (668/685) Candidatus R. sp. An sinensis | KU586118 | |||||
| 96.4% (646/670) Candidatus R. sp. Cx. tritaeniorhynchus | KU586123 | |||||
| 17kDa | A12.2638 | Culex pipiens | 410 | 89.3% (367/411) R. helvetica | LN794217 | NA |
| A12.2646 | Mansonia uniformis | 98.0% (402/410) R. monacensis | LN794217 | |||
| 100.0% (394/394) Rickettsia sp. Tx140 | EF689738 | |||||
| A12.2639 | Culex pipiens | 89.3% (367/411) R. helvetica | LN794217 | |||
| A12.2784 | Culex pipiens | 89.3% (367/411) R. helvetica | LN794217 | |||
| A12.2856 | Aedes esoensis | 89.3% (367/411) R. helvetica | LN794217 | |||
| A12.3106 | Aedes esoensis | 89.3% (367/411) R. helvetica | LN794217 | |||
| A12.3271 | Aedes esoensis | 89.3% (367/411) R. helvetica | LN794217 | |||
| ompB | A12.2638 | Culex pipiens | 640 | 85.0% (192/226) R. hoogstraalii | EF629536 | ≥99.2% |
| A12.2646 | Mansonia uniformis | 690 | 94.1% (649/690) R. hoogstraalii | EF629536 | ||
| 91.1% (626/687) Rickettsia sp. Anopheles gambiae | JN620080 | |||||
| 90.7% (514/567) Rickettsia sp. Culex pipiens | KU761260 | |||||
| A12.2639 | Culex pipiens | - | - | |||
| A12.2784 | Culex pipiens | 646 | 85.3% (198/232) R. hoogstraalii | EF629536 | ||
| A12.2856 | Aedes esoensis | 646 | 85.3% (198/232) R. hoogstraalii | EF629536 | ||
| A12.3106 | Aedes esoensis | 646 | 85.3% (198/232) R. hoogstraalii | EF629536 | ||
| A12.3271 | Aedes esoensis | 649 | 85.5% (201/235) R. hoogstraalii | EF629536 | ||
| gltA | A12.2638 | Culex pipiens | 1004 | 97.8% (982/1004) R. bellii | CP000087 | ≥99.9% |
| 100% 1004/1004) R. bellii WHANSA-97_An. sinensis | KU586331 | |||||
| A12.2646 | Mansonia uniformis | 995 | 96.5% (960/995) R. asembonensis | |||
| 96.7% (695/719) Rickettsia sp. Anopheles melas | JQ354961 | |||||
| 96.5% (920/953) Rickettsia sp. Anopheles gambiae | JN620082 | |||||
| 96.1% (366/381) Rickettsia sp. Culex pipiens | KU761259 | |||||
| 94.7% (942/995) R. monacensis_Cx. quinquefasciatus | KU586332 | |||||
| A12.2639 | Culex pipiens | 1000* | 85.4% (854/1000) Rickettsia felis | CP000053 | ||
| A12.2784 | Culex pipiens | 84.9% (849/1000) R. bellii | CP000087 | |||
| A12.2856 | Aedes esoensis | 96.5% (920/953) Rickettsia sp. Anopheles gambiae | JN620082 | |||
| A12.3106 | Aedes esoensis | |||||
| A12.3271 | Aedes esoensis | |||||
| sca4 | A12.2638 | Culex pipiens | - | - | ≥99.3% | |
| A12.2646 | Mansonia uniformis | 1681 | 92.3% (1559/1689) R. felis | CP000053 | ||
| 98.5% (326/331) Rickettsia sp. Culex pipiens | KU761261 | |||||
| 81.8% (516/631) Rickettsia sp. Anopheles gambiae | JN620081 | |||||
| A12.2639 | Culex pipiens | - | - | |||
| A12.2784 | Culex pipiens | - | - | |||
| A12.2856 | Aedes esoensis | - | - | |||
| A12.3106 | Aedes esoensis | - | - | |||
| A12.3271 | Aedes esoensis | - | - |
NA: not applicable—Not amplified
*-gltA sequences from the five pools were identical represented (A12.3271 pool)