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. 2017 Nov 20;12(11):e0188006. doi: 10.1371/journal.pone.0188006

Fig 4. Vasopressin induced trafficking of FXYD1 in wild type IMCD in vivo.

Fig 4

Wild type mice were injected with dDAVP (1 μg/kg) and sacrificed at 0 time (a-c), 4 hours (d-f), and 16 hours (g-i) post-injection. Fixed kidneys were sectioned, and slides were stained with antibodies against AQP2 (a,d,g) and PLM-C1 antibodies against FXYD1 (b,e,h). dDAVP stimulated the trafficking of FXYD1 from an intracellular location towards apical membrane. Co-localization of FXYD1 and AQP2 at apical membrane is seen in yellow after 4 hours (f). This figure is representative of 3 independent experiments. Nuclei were labeled with TO-PRO-3. Bars, 10 μm.