Fig 2.

Effects of SO₂ donor on the contents of superoxide anion (⋅O₂^–) (A), hydrogen peroxide (H₂O₂) (B) and malondialdehyde (MDA) (C) in GA₃-treated barley aleurone layers. Aleurone layers are treated with 20 μM GA₃ + H₂O (CK) or 20 μM GA₃ + 50 μM SO₂ donor (SO₂) for 60 h. Aleurone layers treated for 24 and 48 h are incubated with DCHF-DA and observed by fluorescence microscopy (D). The relative fluorescence intensity of images analyzed by ImageJ is shown in (E). Data are expressed as means ± SD of three independent experiments with three replicates of 15 aleurone layers per treatment. The symbols * and ** in this figure and following ones stand for significant difference at P < 0.05 and P < 0.01 between the control and SO₂ treatment, respectively. Bar, 100 μm.