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. 2017 Sep 14;158(11):3738–3753. doi: 10.1210/en.2017-00098

Figure 2.

Figure 2.

Yap and Taz knockdown efficiency in male Yapflox/flox;Tazflox/flox;Nr5a1cre/+ mice. (A) PCR genotype analyses for Yap and Taz performed on adrenal glands from 10-week-old males of the indicated genotypes. Bands corresponding to the floxed and Cre-recombined alleles are indicated. (B) qRT-PCR analysis of Yap and Taz mRNA levels in the adrenal glands of 10-week-old males of the indicated genotypes (n = 6 animals per genotype). All data were normalized to the housekeeping gene Rpl19 and are expressed as means (columns) ± standard error of the mean (error bars). Asterisks indicate significantly different from control (**P < 0.01; ****P < 0.0001). (C, D) Immunohistochemical analysis of YAP expression in adrenal glands from 10-week-old male (C) Yapflox/flox;Tazflox/flox and (D) Yapflox/flox;Tazflox/flox;Nr5a1cre/+ mice. (E, F) Immunohistochemical analysis of TAZ in adrenal glands from 10-week-old male (E) Yapflox/flox;Tazflox/flox and (F) Yapflox/flox;Tazflox/flox;Nr5a1cre/+ mice. Scale bar in (F) is valid for all images.