Table 1.
Source of Antibodies Used in This Study
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer (Catalog No.) | Species Raised in; Monoclonal or Polyclonal | Dilution Used | RRID |
|---|---|---|---|---|---|---|
| STAT5b | Synthetic nonphosphopeptide derived from human STAT5b around the phosphorylation site of serine 731(A-P-S-P-A) | Anti-Stat5b | Abcam (ab52162) | Rabbit; polyclonal | 1:1000 | AB_882716 |
| STAT5b (phospho S731) | Synthetic phosphopeptide based on human STAT5B around the phosphorylation site of serine 731 (A-P-SP-P-A) | Anti-pStat5b, pS731 | Abcam (ab52211) | Rabbit; polyclonal | 1:500 | AB_2196931 |
| LC3/MAP1LC3A | A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25 and 121) | Anti-LC3 | Novus Biologicals (NB100-2331) | Rabbit; polyclonal | 1:500 | AB_10001955 |
| GAPDH | Purified rabbit muscle GAPDH (whole molecule) | Anti-GAPDH | Ambion (AM4300) | Mouse; monoclonal | 1:5000 | AB_437392 |
| Goat IgG | Not applicable | Goat anti-rabbit IgG-HRP | Santa Cruz Biotechnology (SC2004) | Goat; polyclonal | 1:10,000 | AB_631746 |
| Goat IgG | Not applicable | Goat anti-mouse IgG-HRP | Santa Cruz Biotechnology (SC2005) | Goat; polyclonal | 1:10,000 | AB_631736 |
Abbreviations: LC3/MAP1LC3A, microtubule-associated proteins 1A/1B light chain 3A; RRID; Research Resource Identifier; Stat5b, signal transducer and activator of transcription 5B.