Skip to main content
. 2017 Jul 21;158(11):3778–3791. doi: 10.1210/en.2017-00159

Figure 2.

Figure 2.

MEF2C is necessary for Mmp13 basal and PTH-stimulated promoter activity and associates with the Mmp13 promoter. (A) UMR 106-01 cells were plated in 12-well plates and transfected with 100 ng of pGL2-Mmp13 (−148 rat Mmp13 promoter) and 60 nM of Mef2c siRNA or scrambled siRNA as indicated. Cells were treated with or without 10−8 M PTH-(1-34) for 6 hours. Firefly luciferase activity was measured, and relative light units (RLU) were normalized to the amount of total protein. Data shown are mean ± SE. ***P < 0.001 vs control or PTH-treated scrambled set, n = 3. (B) UMR 106-01 cells were treated with or without PTH for the indicated time, and collected for ChIP assays with antibodies against MEF2C, or IgG as a negative control. DNA was amplified by real-time PCR using primers for the rat Mmp13 proximal promoter region (−205/−4). Data shown are mean ± SE. *P < 0.05, **P < 0.01 vs control at each time point, n = 3. N.S., not significant. (C) The cells were treated with or without PTH for 4 hours and collected for ChIP assays with antibodies against MEF2C, or IgG as a negative control. The primers used for real-time PCR were from the Mmp13 proximal (−205/−4), or the distal (−1240/−969) promoter regions. Data shown are mean ± SE. **P < 0.01 vs control, n = 3.