Figure 3.
MEF2C associates with the AP-1 site on the Mmp13 promoter. (A) The nuclear extracts from Mef2c-transfected UMR 106-01 cells treated with 10−8 M PTH (1-34) for 2 hours were subjected to gel mobility shift analyses using biotin-labeled oligomers corresponding to RD or AP-1 sequences of the rat Mmp13 promoter as shown in Table 1. Binding specificity of the complex was verified in competition experiments performed with a 200-fold molar excess of unlabeled oligomers (lanes 4 and 9). An antibody against MEF2C was added to the incubations in lanes 5 and 10. (B) UMR 106-01 cells were transfected with 60 nM Mef2c siRNA or scrambled siRNA. The nuclear extracts from the cells treated with PTH for 2 hours were subjected to gel mobility shift analysis.