Figure 8.
After PTH treatment (4 hours), the binding between MEF2C and p300 and Runx2 to the Mmp13 promoter is increased. UMR 106-01 cells were treated with or without 10−8 M PTH for 4 hours. Cell lysates were collected for ChIP-reChIP assays with antibody against MEF2C, or IgG as a negative control for the first ChIP, and antibody to (A) p300, (B) CBP, (C) Runx2, or IgG was used for the second immunoprecipitation. DNA was amplified by real-time PCR using primers for the rat Mmp13 proximal promoter region. Data shown are mean ± SE. *P < 0.05, **P < 0.01 vs control, n = 3.