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Restraint stress inhibits pulsatile LH in OVX mice. Profiles of circulating LH in (A–C) three representative OVX no-stress control mice and (D–F) three OVX mice exposed to restraint stress for 90 minutes beginning at time 0. Pulse peaks are indicated by open symbols. The period of treatment is depicted by gray shading. Tail-tip blood samples (3 μL) were collected every 5 minutes by pipette, and LH pulses were detected by DynPeak pulse algorithm.
