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. 2001 Aug 14;98(18):10320–10325. doi: 10.1073/pnas.171060098

Figure 3.

Figure 3

Neoplastic lesions in Pten+/− uteri have lower levels of Pten, and higher active Akt. (A) Loss of heterozygosity in hyperplastic lesions of the endometrium. Products from wild-type and mutant Pten alleles are amplified in a duplex reaction. Controls consist of products generated from tail DNA isolated from Pten heterozygous (lane 1) and wild-type (lane 2) mice. Lanes 3, 4, and 5 are amplified products from microdissected, hyperplastic endometrial lesions from three Pten heterozygous mice at 32 weeks of age. Loss of the wild-type Pten allele is present in one lesion (lane 3), and both alleles are retained in the other two lesions (lanes 4 and 5). (B) Loss of heterozygosity in Pten+/− adrenals. Adrenal DNA was prepared from six Pten+/− mice. After probing the wild-type (wt) and mutant alleles (mut) (arrowheads), we observed that five of the six Pten+/− adrenals had undergone loss of heterozygosity. Control (+/−) and wild-type DNA (+/+) were prepared from tails. (C–E) Transition zone in Pten+/−-transformed uterine cysts. Slides were stained with hematoxylin/eosin (C), rabbit polyclonal anti-PTEN (D), and rabbit polyclonal anti-phospho-AKT (Ser-473) (E). (Magnification, ×600.) (F and G) Altered PTEN and phospho-AKT expression are detected in the adrenal medulla. Reduced PTEN staining correlates with transformation and phospho-AKT staining. (F) A small representative focus of reduced PTEN expression in a Pten+/− adrenal medulla. Notice that most PTEN staining within the medullary cells occurs in the nucleus. (G) A small focus of increased phospho-AKT staining correlates with reduced PTEN expression. (Magnification, ×600.) Cortex (C) stains nonspecifically for PTEN and phospho-AKT.