Figure 1.

Laminins Are Required for Proper Hemocyte Migration around the Hindgut and Over the VNC and for Lamellipodia Dynamics and Persistence

(A–F) Stills taken from live imaging of GFPMoe-expressing hemocytes migrating around the hindgut in control (A–C) and LanB1 mutant embryos (D–F).

(G) Tracking individual hemocytes reveals a significant decrease in the velocity of hemocytes from LanB1 mutant embryos (p < 0.001).

(H and I) Graph showing a significant decrease in the velocity of hemocytes from LanB1 embryos compared to control on both the (H) ventral (p < 0.0001) and (I) dorsal sides of the VNC (p < 0.01).

(I and J) Hemocytes from control (I) and LanB1 (J) embryos.

(K and L) Graphs showing the lamellipodial area of (K) control (n = 6) and (L) LanB1 (n = 7) hemocytes measured at 30-s intervals over a 30-min time period.

(M and N) Average lamellipodial area (M) and lamellipodial area change (N) per hemocyte from control and LanB1 embryos (n = 6 and 7 hemocytes from 3 different embryos per genotype, respectively).

(O and P) Radial diagrams illustrating lamellipodia distribution in control (O) and LanB1 embryos (P).

Scale bars represent 50 μm (A–F) and 20 μm (I and J).