Key resource table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (Saccharomyces cerevisiae) |
Ltv1-FTpA;Tsr1shuffle | This paper | NA | Ltv1-FTpA- Genomic copy of Ltv1 tagged at the c-terminus with FLAG-TEV-proteinA tag; Tsr1 shuffle- genomic copy of Tsr1 deleted and rescued with a plasmid with a wild-type copy |
| genetic reagent (plasmids used for expression in Saccharomyces cerevisiae) |
YCplac111-Tsr1-FTpA; YCplac111-Tsr1 R54D, K55D,K57D-FTpA; YCplac111-Tsr1 R54D, K55D,K59D-FTpA; YCplac111-Tsr1 R54D, K57D,K59D-FTpA; YCplac111-Tsr1 K55D, K57D,K59D-FTpA; YCplac111-Tsr1 R54D, K55D,K57D,K59D-FTpA; YCplac111-Tsr1-GFP; YCplac111-Tsr1 R54D, K55D,K57D,K59D-GFP; YCplac111- Tsr1DC86-FTpA |
This paper | NA | Plasmids containing wild-type Tsr1 or the described point mutants. Expression is under the control of the Tsr1 promoter, and are tagged at the c-terminus with either FLAG-TEV-proteinA or GFP. |
| software, algorithm | EM-TOOLS | TVIPS GmbH | NA | http://www.tvips.com/imagingsoftware/em-tools/ |
| software, algorithm | MotionCorr2.1 | https://doi.org/10.1038/nmeth.4193 | NA | http://cryoem.ucsf.edu/software/driftcorr.html |
| software, algorithm | GCTF | https://doi.org/10.1016/j.jsb.2015.11.003 | NA | http://www.mrclmb.cam.ac.uk/kzhang |
| software, algorithm | Gautomatch | public | NA | http://www.mrclmb.cam.ac.uk/kzhang |
| software, algorithm | Relion-2 | https://doi.org/10.7554/eLife.18722 | NA | http://www2.mrclmb.cam.ac.uk/relion/index.php |
| software, algorithm | Phenix suite (phenix.real_space_ refine, molprobity) |
Python-based Hierarchical ENvironment for Integrated Xtallography |
RRID:SCR_014224 | https://www.phenix-online.org/ |
| software, algorithm | CCP4 (LIBG, ProSMART, Refmac5, COOT) |
Collaborative Computational Project No. 4 Software for Macromolecular X-Ray Crystallography |
RRID:SCR_007255 | http://www.ccp4.ac.uk/ |
| software, algorithm | UCSF Chimera | UCSF Resource for Biocomputing, Visualization, and Bioinformatics |
RRID:SCR_004097 | http://www.cgl.ucsf.edu/chimera/ |
| software, algorithm | Pymol | PyMOL Molecular Graphics System, Schrödinger, LLC |
RRID:SCR_000305 | https://pymol.org/ |