Figure 6.

Respiration and ROS Generation in Eif6 Heterozygote Muscle

(A) Typical oxygen utilization dynamics of muscle tissue before and after addition of ADP to the medium.

(B) Respiratory control ratio (ratio of respiration state 3 to state 4) is significantly lower in Eif6^+/− (n = 8) muscle compared with WT muscle (n = 8).

(C) P:O ratio is not significantly altered in Eif6 heterozygous muscle.

(D) Mitosox (405-nm) signal (superoxide abundance) over time in response to electrically stimulated contraction in WT and Eif6^+/− FDB muscle fibers. Black bars indicate periods of stimulation. Repeated-measures p value: WT versus WT stimulated, p = 0.0016, Eif6^+/− versus Eif6^+/− stimulated, p = 0.38, WT versus Eif6^+/− non-stimulated, p < 0.01.

(E and F) SOD1 (E) and SOD2 (F) protein expression measured by western blot in Eif6^+/− muscle.

Data are represented as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01.