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. 2017 Nov 7;21(6):1655–1666. doi: 10.1016/j.celrep.2017.10.053

Figure 2.

Figure 2

PTEN Prevents Endosome-to-Plasma Membrane Recycling of GLUT1

(A) A representative western blot showing the shRNA-mediated knockdown of PTEN in HeLa cells.

(B) PTEN-depleted HeLa cells were fixed and imaged using a confocal microscope after staining with antibodies against GLUT1. Representative images are shown, Scale bars, 5 μm.

(C) HeLa cells were transduced with PTEN shRNAs alone or in combination with SNX27 shRNAs, and a representative western blot showing the knockdown of PTEN and SNX27 is shown.

(D) HeLa cells transduced with the indicated shRNAs were fixed and imaged using a confocal microscope after staining with antibodies against GLUT1. Representative images are shown. Scale bars, 5 μm.

(E) Quantification of plasma membrane (PM)-to-cytoplasmic (Cyt) ratio of GLUT1 intensity per each cell was plotted. Error bars indicate SD (n = 100 cells). ∗∗∗p < 0.001 by Student’s t test.

(F) HeLa cells transduced with PTEN shRNA were transfected with shRNA-resistant PTEN WT or the indicated mutants and then processed for staining with GLUT1. Representative images are shown. Scale bars, 5 μm.

(G) Quantification of PM-to-Cyt ratio of GLUT1 intensity per each cell was plotted. Error bars indicate SD (n = 40 cells). ∗∗∗p < 0.001 by Student’s t test.